Title: Novel Insights in the Regulation of Phosphatidylserine Exposure in Human Red Blood Cell Authors: Wesseling, M.C. Wagner-Britz, L. Nguyen, D.B., (...) Kaestner, L. Bernhardt, I. Keywords: Ca2+ content;Flow cytometry;Fluorescence imaging;Phosphatidylserine exposure;Protein kinase C;Red blood cells Issue Date: 2016 Publisher: S. Karger AG Citation: Scopus Abstract: In previous publications we were able to demonstrate the exposure of phosphatidylserine (PS) in the outer membrane leaflet after activation of red blood cells (RBCs) by lysophosphatidic acid (LPA), phorbol-12 myristate-13acetate (PMA), or 4-bromo-A23187 (A23187). It has been concluded that three different mechanisms are responsible for the PS exposure in human RBCs: (i) Ca2+-stimulated scramblase activation (and flippase inhibition) by A23187, LPA, and PMA; (ii) PKCα activation by LPA and PMA; and (iii) enhanced lipid flip flop caused by LPA. Further studies aimed to elucidate interconnections betw
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